Manufacturing Solutions
Blaze Vector Production
Forge provides Blaze Vector Production to help academic scientists and gene therapy developers access turnkey, scalable, research-grade vector production without a long-term commitment.
Overview
- Our proprietary Ignition Cells have a clear, well-documented history and are serum-free HEK293s optimized for yield and consistency in the suspension system.
- Open licensing and royalty-free usage.
- Established process for any AAV serotype.*
- Upfront communication and competitive pricing.
- Fast turnaround time in 6-8 weeks.
- Process development to perform experiments on novel engineered capsids.
- Experienced team with decades of AAV manufacturing experience.
- Dedicated client support throughout your journey with Forge.
Scales
Small - 1 L
Mid - up to 10 L
Large - up to 50 L
Blaze Process
Step 1: Research-grade plasmid manufacturing
Step 2: Proprietary Ignition Cells™ are serum-free HEK293s optimized for yield and consistency
Step 3: Triple Transfection model and hold
Step 4: Cell Lysis & Nuclease
Step 2: Proprietary Ignition Cells™ are serum-free HEK293s optimized for yield and consistency
Step 3: Triple Transfection model and hold
Step 4: Cell Lysis & Nuclease
Step 6: Bulk harvest material is clarified by depth filtration
Step 7: Affinity Chromatography
Step 8: Cesium Chloride Ultracentrifugation Gradient
Step 9: Dialysis
Step 10: .2uM Filtration
Step 11: Fill & Finish Drug Product
Step 7: Affinity Chromatography
Step 8: Cesium Chloride Ultracentrifugation Gradient
Step 9: Dialysis
Step 10: .2uM Filtration
Step 11: Fill & Finish Drug Product
*Common Serotypes
- AAV1
- AAV2
- AAV2.7m8
- AAV3B
- AAV5
- AAV6
- AAV6.2
*Common Serotypes
- AAV7
- AAV8
- AAV9
- AAVrh10
- AAVrh74
- Anc80
- Plus novel serotypes